DNA barcoding is a taxonomic method that uses a short genetic marker in an organism’s DNA to identify it as belonging to a particular species. In our daily lives, various fishes can be seen. Fish with different appearances, sizes and habitats have distinguishing phenotypes. I was interested in determining if a taxonomic analysis using DNA barcoding would match with their observed phenotypes. So in this project, I chose eight common fishes from the market: six are used for eating and two are ornamental fishes. Some are with similar shapes, while some have different living environments. By extracting DNA and analyzing their base sequences, the extents of relation between these fishes would be found out.