Suzhou Creek, also called the Wusong River, is a river that flows into Huangpu River. The origin of it is the Lake Tai, which locates in Jiangsu province. As the Suzhou Creek flows through this wide range, the genetic relationship of plants along Suzhou creek could be a feasible topic to be explored by utilizing DNA barcoding technique. Here, we collected several plant specimens from two specific locations on the river bank. Comparing PHYLIP ML to construct systematic evolutionary relationship between allopathy plant specimens. The eliminated aim was to demonstrate the influence of water flow on plant migration by comparing morphological and genetic similarities in the herbarium specimens.