This experiment aims to explore how NO2 affects leaf biodiversity in Black Rock Forest and Central Park. The samples will be collected from Black Rock Forest and Central Park. Then, rapid DNA isolation will be used to decode and determine the species of the leaves. Lastly, the biodiversity and NO2 levels will be compared to determine their correlation. Through this experiment, we hope to prove that NO2 has effects on the biodiversity of trees, and therefore, more effects should be used to reduce NO2 near areas with large populations of trees.