Central Park, an iconic urban green space, is a habitat hosting a rich diversity of microorganisms, which play pivotal roles in ecosystem functions and health. Soil pH, a fundamental environmental factor, has the potential to significantly impact these microorganisms. In this DNA barcoding project, we investigate the relationship between soil pH and microorganisms’ biodiversity within Central Park. By collecting soil samples from two contrasting locations, characterized by varying pH levels, we aim to uncover the influence of pH on microbial diversity and composition. This project seeks to understand how soil pH can shape the ecosystem of Central Park's microorganisms and contribute to our understanding of DNA barcoding.