Fungi are critical to ecosystem health, functioning as decomposers and symbionts that contribute to nutrient cycling and biodiversity. This study examines fungal biodiversity in two distinct environments within Westchester County, NY: Cranberry Lake Preserve, a protected natural area, and Twin Lakes Park, a recreational area with higher human activity. By collecting and analyzing fungal samples from each location, this project aims to quantify species richness and composition, comparing fungal communities based on land use differences. DNA barcoding, specifically targeting the ITS region, along with field guides and iNaturalist, will be used to accurately identify fungal species. Findings from this study will help characterize the diversity of fungi within these ecosystems, providing a scientific basis for understanding how environmental factors influence fungal communities.