This project examines genetic variation in white oak trees across urban Central Park and semi-natural Black Rock Forest. DNA sequencing will identify genetic markers unique to each population, with Central Park trees potentially showing adaptive traits to urban conditions. Soil composition, including factors like sunlight exposure and air quality, will also be analyzed due to soil’s role in influencing plant adaptation and health. Differences in soil properties between sites may correlate with genetic variations observed in the trees. By comparing genetic and environmental data, we aim to uncover adaptive traits in urban white oaks and understand how urbanization impacts tree genetics and soil ecology. These findings could inform urban tree management, enhance biodiversity, and promote ecosystem health in cities. This approach highlights the importance of understanding genetic and ecological responses to urban environments for sustainable urban forestry practices.