Project:

Sanger sequencing-based DNA barcoding: Detection of Commercial Dog Food Mislabeling

Students:

Jiaming Ni, Zhifan Wu, Yanxuan Shi, Zixuan He

School:

jiangsu province liangfeng high school international college; Kangchiao international school; Ulink High School of Suzhou Industrial Park; Soochow University High School Sino-Canada Program, Suzhou, China

Mentors:

Xinyue Wang

Abstract:

In our case of study, we set up a comparison between authentic fresh meat (chicken meat and beef) bought from local grocery and dog snacks bought from pet shops(4 types of duck meat, chicken meat, rabbit rib, beef, and two types of mix products). We isolated the cytochrome c oxidase I (COI) gene from all the samples using Silica DNA Isolation at the first, run the PCR and then, applying Sanger Sequencing to acquire the DNA barcodes of both the fresh meat and dog foods. Comparison groups of both fresh meat and dog food enables the detection of mislabeling.

Poster:

Team samples: