Urban tree management relies on accurate species identification to determine which trees are most ideal for planting in New York City. Although New York City has a policy of planting only male Gingko biloba trees, this dioecious species has the unique ability to change sexes and change from male to female after being planted. In contrast, Fraxinus pennsylvanica is also a dioecious species, but lacks this ability to change sexes. This project uses DNA barcoding of the chloroplast gene rbcL from male and female samples of both the Gingko Biloba, Fraxinus pennsylvanica, and existing DNA on DNA Subway to determine if there is a genetic pattern present in the Gingko Biloba that correlates with its ability to change sexes. Findings from this project will inform future decisions to plant Gingko Biloba trees in New York City.