Urban parks often contain a mix of native and introduced plant species, some of the introduced species may threaten local biodiversity. This project aims to assess whether widely distributed plants in Suzhou Bailu Garden pose a potential risk of invasion to the park’s ecosystem. During the process, we use Chelex DNA isolation,Silica DNA isolation (for samples that failed the first PCR) and PCR. The samples are sequenced by GENEWIZ and Oxford Nanopore Technology. I expect to identify the species and origin of the samples and assess their risk of invasion by using PHYLIP to explore their relationship with known invasive species. It turns out that the park is being invaded. Among the 13 samples, 10 are non-native.Cuscuta campestris,Verbena bonariensis and Compositae plants such as Conyza bonariensis and Oligoneuron have great potential for being invasive.