As microbial communities within arachnids are compared to heavy metal concentrations, information about environmental conditions from which samples were collected will be revealed. These environmental conditions may prompt further investigations of specific environments as the correlation between heavy metals present in arachnid abdomen and microbiota present in arachnid abdomen are examined. Samples will be collected using transects and quadrats. Microbial communities will then be determined using 16S rDNA. ICP-MS will be used to detect heavy metals. It is expected that arachnids with similar heavy metals will have similar microbial communities present and can then be grouped together when data is displayed.