Using DNA barcoding, five different species from the Phrynosomatidae family are to be sequenced and identified to be able to construct a phylogenetic tree. The Phrynosomatidae family is a diverse group of lizards and an important model for research in ecology and evolution in the southwest of America and Mexico. Multiple samples will be used for each species to help determine if any undiscovered species might be present. Building a phylogenetic tree will allow us to possibly discover cryptic species and understand the biogeographical patterns of the areas which the samples came from. Finally, formal species delimitation analyses will be used to test for new species.