DNA barcoding is widely used to identify species. In this research, we took several samples from the developed area and the countryside and used DNA barcoding technology to identify them, attempting to gain an insight into the ecosystems of the city and countryside and find the differences between them. We used both Silica and Rapid methods to extract DNA, sequenced them with the help of AZENTA, and used to research the relationship between the sample organisms and other creatures, then we would know what the sample exactly is, or at least what it is similar to. We found that the biodiversity is more abundant in the countryside, and there are some invasive species in both the city and the countryside. We proposed several ways to control the invasive species, including chemical methods and biological methods.