Project:

The Phylogenetic Analysis for Bambusoideae Species Based on DNA Barcoding

Students:

Wenxin Zhang, Zhongyu Lu, Zhimeng Bao, Letong Xu

School:

Beijing Academy;Wellington China International Shanghai;WLSA Shanghai Academy;Xi'an gaoxin number two middle school, Suzhou, China

Mentors:

Xinyue Wang

Abstract:

The harsh climate and other human factors have put Bambusoideae species into a dangerous situation, emphasizing the importance and the urgency of improving the database and its classification. Our research aims to find the phylogenetic relationships between bamboos to provide evidence for related preserving measures from the molecular aspect. Partial fragments of the isolated DNA from 20 Bambusoideae species will be amplified by PCR (polymerase chain reaction) using 3 primers (rbcL, matK, ITS2) and sent to Azenta, China for sequencing. The best PCR results have been acquired using the rbcL primer, showing that the Bambusoideae species in the Surging Waves Pavilion can be separated into 2 groups and 3 subgroups.

Poster:

Team samples: