The CO1 gene provides a reliable way of identifying species, even ones that are similar or are very complex. This accuracy is essential for documenting and understanding the true diversity of moths and butterflies. Utilizing the CO1 gene to navigate the biodiversity of Lepidoptera is a powerful tool that enhances our understanding of moth and butterfly diversity, as well as informs conservation efforts, helps ecological studies, and clarifies taxonomic relationships. This research is crucial for maintaining the ecological balance and health of ecosystems where moths and butterflies play a vital role.