Urban ponds serve as vital ecosystems in New York City, supporting diverse microbial and fungal populations that play crucial roles in nutrient cycling and water quality. However, the impact of these organisms, particularly fungi, on pond health remains underexplored. This research aims to investigate the diversity and ecological roles of fungi in New York City's ponds, focusing on their potential as bioindicators of water quality. By analyzing fungal populations across multiple pond sites, we seek to determine the correlation between fungal diversity and water health parameters such as pH, dissolved oxygen, and pollutant levels. We hypothesize that fungal presence and diversity will be linked to key water quality metrics, with certain fungal species serving as indicators of high nutrient levels or water contamination. Additionally, we aim to detect potentially pathogenic or invasive fungal species that could pose ecological risks. This study utilizes DNA barcoding techniques, specific