Project:

Shelter Island Sequencing

Students:

Michael Kotula, Nathan Cronin, Robert Beckwith

School:

Shelter Island Union Free School District, Suffolk

Mentors:

Daniel Williams

Abstract:

Advancing molecular biology requires efficient and accurate methods for sequencing DNA and RNA. Sanger sequencing, the most widely used technique, offers high accuracy (99.99%) but involves a complex and costly process requiring unique primers for each target region. In contrast, nanopore sequencing generates unique electrical signals for nucleotides passing through a nanopore, enabling high-throughput sequencing at lower costs. While Sanger is ideal for small-scale projects, nanopore sequencing is more cost-effective for large-scale applications. To compare these methods, we propose using DNA Subway to analyze sample sequences prepared with both techniques, assessing their similarities, differences, and potential applications.

Poster:

Team samples: