We are investigating the Twin Lakes, and Mill Pond because its mix of freshwater ponds, lakes, and moist soil habitats supports a high diversity of small invertebrates that are often underrepresented in genetic databases. By collecting specimens from these environments, we hope to help identify and barcode species that are unique to or abundant in our desired locations for collecting bugs. Our collection methods will include both passive traps—such as pitfall traps lined with plastic—and active sampling with nets for aquatic invertebrates. After collection, the specimens will be frozen for preservation and prepared for DNA extraction and sequencing. Through this process, we aim to contribute valuable data on Long Island’s invertebrate diversity and support broader efforts in conservation and species identification.