This Project uses MC's DNA barcoding to survey the genetic diversity of fireflies on Long Island. Fireflies, beetles known for their bioluminescence, are experiencing documented population declines worldwide, and their local species identification is now crucial for conservation. We are motivated to build a molecular inventory of the firefly species existing in local habitats. We will utilize samples provided by the DNALC. Methods will involve DNA isolation through standard silica-based volume purification and PCR amplification of the Cytochrome c oxidase I gene region. We expect to confirm the presence of common Long Island species like Photinus pyralis and uncover novel sequence variations or cryptic species within the local Lampyridae population, which could point to habitat fragmentation impacts