Pollinators play a crucial role in both natural ecosystems and human agriculture, yet their diversity is threatened by urbanization, habitat loss, and artificial light. This study will investigate how diurnal and nocturnal pollinator communities differ between urban gardens and natural meadows on Long Island. We will collect insects during the day and night. Specimens will be identified through DNA barcoding to accurately assess species richness and community composition. We expect natural meadows to host higher overall diversity, while urban gardens will support fewer, more tolerant species, particularly with reduced nocturnal activity due to light exposure. The findings will highlight the importance of both day and night active pollinators and inform urban conservation strategies.