Resources
Classroom Downloads
All Programs
Protocol: Using DNA Barcodes to Identify and Classify Living Things [PDF]
Introduction, experimental method, bioinformatics, and supporting resources for using DNA barcoding to identify plants or animals – or products made from them.
Introduction to DNA Barcoding [Powerpoint]
Introduction, experimental method, bioinformatics, and supporting resources for using DNA barcoding to identify plants or animals – or products made from them.
Silica DNA Isolation Worksheet [PDF]
Introduction, experimental method, bioinformatics, and supporting resources for using DNA barcoding to identify plants or animals – or products made from them.
Data Collection Sheet [MS Word]
Data Collection Sheet [PDF}
Suggested Organism Sampling Techniques [PDF]
Adding Sample Information to the Barcode Sample Database [PDF]
(for general use)
Adding Sample Information to the Barcode Sample Database [PDF]
(for Barcode Long Island and Urban Barcode Project Student Teams)
Protocol for uploading gel images of PCR amplified DNA samples to the Barcode Sample Database [PDF]
(for Barcode Long Island and Urban Barcode Project Student Teams)
Instructions for Sample Documentation [PDF]
(for Barcode Long Island and Urban Barcode Project Student Teams)
Adapted AmScope Microscope Guide [PDF]
(for Barcode Long Island and Urban Barcode Project Student Teams)
Taxonomic Resources [PDF]
Electrophoresis Gel Tracking Sheet [MS Excel]
DNA Subway Guide [PDF]
Sequence Alignment Exercise [PDF]
Evolution of the Chocolate Bar: A Creative Approach to Teaching Phylogenetic Relationships Within Evolutionary Biology [PDF]
Photo/Video Release Form, Opt-out [PDF]
References
- Baker CS, Steel D, Choi Y, Lee H, Kim KS, Choi SK, et al. (2010) Genetic evidence of illegal trade in protected whales links Japan with the US and South Korea. Biology Letters 6:647-650.
- Cecchetto, M., Di Cesare, A., Eckert, E., Moro, I., Fontaneto, D., Schiaparelli, S. (2022). A Metabarcoding Protocol to Analyze Coastal Planktic Communities Collected by Desalination Plant Filters: From Sampling to Bioinformatic Exploratory Analyses. In: Verde, C., Giordano, D. (eds) Marine Genomics. Methods in Molecular Biology, vol 2498. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2313-8_8
- DeSalle R, Egan MG and Siddall M.(2005) The unholy trinity: taxonomy, species delimitation and DNA barcoding. Philosophical. Transactions of the Royal Society B 360, 1905-1916.
- Ficetola GF, Miaud C, Pompanon F, Taberlet P. (2008) Species detection using environmental DNA from water samples. Biology Letters 4(4):423-425.
- Hebert PDN, Cywinska A, Ball SL, DeWaard JR. (2003) Biological identifications through DNA barcodes. Proceedings of the Royal Society B: Biological Sciences 270(1512):313-321.
- Hebert PDN, Gregory TR. (2005) The promise of DNA barcoding for taxonomy. Syst Biol 54(5):852-859.
- Hebert PDN, Penton EH, Burns JM, JAnzen DH, Hallwachs W (2004) Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proceedings of the National Academy of Sciences 101(41):14812-14817.
- Hollingsworth PM. (2007) DNA Barcoding: Potential Users. Genomics, Society and Policy 3(2):44-474.
- Huang J, Xu Q, Sun, ZJ, Tang GL and Su, ZY. (2007) Identifying earthworms through DNA barcodes. Pedobiologia 51, 4:301-309
- Hubert N, Hanner R, Holm E, Mandrak NE, Taylor E, Burridge M, et al. (2008) Identifying canadian freshwater fishes through DNA barcodes. PLoS ONE 3(6): e2490.
- Leavitt S.D, Fernandez-Mendoza F, Perez-Ortega S, Sohrabi M, Divakar P.K, et al. (2013) DNA barcode identification of lichen-forming fungal species in the Rhizoplaca melanophthalma species-complex (Lecanorales, Lecanoraceae), including five new species. MycoKeys 7: 1–22
- Lowenstein JH, Amato G, Kolokotronis S. (2009) The real maccoyii: Identifying tuna sushi with DNA barcodes — contrasting characteristic attributes and genetic distances. PLoS ONE 4(11): e7866.
- Meyer CP, Paulay G. (2005) DNA barcoding: Error rates based on comprehensive sampling. PLoS Biology 3(12): e422.
- Schiaparelli, S., Alvaro, M.C., Cecchetto, M., Guzzi, A. (2022). Barcoding of Antarctic Marine Invertebrates: From Field Sampling to Lab Procedures. In: Verde, C., Giordano, D. (eds) Marine Genomics. Methods in Molecular Biology, vol 2498. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2313-8_9
- Schwartz J (2008) Fish Tale Has DNA Hook: Students Find Bad Labels. New York Times, August 21, 2008.
- Steinke D, Zemlak TS, Hebert PDN. (2009) Barcoding Nemo: DNA-based identifications for the ornamental fish trade. PLoS ONE 4(7): e6300.
- Vernooy R, Haribabu E, Muller MR, Vogel JH, Hebert PDN, Schindel DE, et al. (2010) Barcoding life to conserve biological diversity: Beyond the taxonomic imperative. PLoS Biology 8(7): e1000417.
- Wong EHK and Hanner RH (2008) DNA barcoding detects market substitution in North American seafood. Food Research International 41: 828–837.
Online Tools & Databases
Sample Database
The DNALC’s Barcode Sample database supports our student DNA barcoding research projects.
DNA Subway
Use the Blue Line of DNA Subway to analyze novel DNA sequences generated by a DNA sequencing experiment.
Encyclopedia of Life
The Encyclopedia of Life (EOL) is a free, online collaborative encyclopedia intended to document all of the 1.9 million living species known to science.
Barcode of Life Data (BOLD) Systems
The Barcode of Life Data Systems is designed to support the generation and application of DNA barcode data. The platform consists of four main modules: a data portal, a database of barcode clusters, an educational portal, and a data collection workbench.
International Union for the Conservation of Nature
The International Union for Conservation of Nature and Natural Resources is an international organization working in the field of nature conservation and sustainable use of natural resources.
The IUCN Red List of Threatened Species
The IUCN Red List of Threatened Species™ is the world's most comprehensive inventory of the global conservation status of plant and animal species. It uses a set of criteria to evaluate the extinction risk of thousands of species and subspecies.
