In the environment, biodiversity is very important in the environment. Biodiversity of natural environments can be both positive and negative, with certain physical, mental and social health. Biodiversity is necessary for life in the environment and since the rate of it is declining at a concerning rate, the quality of life of all humans is being threatened. To find the biodiversity of the stream, DNA barcoding will be used, which is finding the sizes of DNA in the specimens to find the species of the organisms. The question that is being proposed is how is biodiversity changing on a yearly basis? The objective of this research is to see how the biodiversity changes on a yearly scale and what creatures are abundant in the environment of the Massapequa preserve.