Longwood Senior High School is partnering with the Open Lab at Brookhaven National Laboratory and Cold Spring Harbor to research the biodiversity of insects as well as the macroinvertebrate population in Carmens River. The Carmans River starts in Middle Island, and flows south about 10 miles to Bellport Bay (1). The Carmans River is both unique as it is a mix of fresh and salt water (2) where many invertebrates thrive. Some examples of native invertebrate species are the Starry Sky Beatle, Spotted Wing Drosophila, Spotted Lantern Fly, and the Jumping Worm along with many others (3). Additionally, some invasive species there are also invasive species such as watercress (Nasturtium officinale), phragmites (Phragmites australis), and Eurasian watermilfoil (Myriophyllum spicatum) within the river (3). The goal of the experiment is to see what invertebrates are invasive and entering into the Carmans River. Invertebrates are collected from Carmans River and their DNA is going to be inspected